2) Both CTmax and heat coma values were significantly different

2). Both CTmax and heat coma values were significantly different between species and were progressively greater from C. antarcticus (30.1 and 31.8 °C), through M. arctica (31.7 and 34.6 °C), to A. antarcticus (34.1 and 36.9 °C) (P < 0.05 Tukey’s multiple range test, variances not equal). A one

month acclimation at −2 °C significantly reduced CTmax and heat coma temperatures compared to individuals maintained at +4 °C in all species (Fig. 2, P < 0.05 Kruskal–Wallis test). A two week acclimation at +9 °C also led to lower (or unchanged – C. antarcticus) CTmax and heat coma temperatures, though this was only significant for the heat coma temperature of A. antarcticus (P < 0.05 Kruskal–Wallis test). Summer acclimatised individuals of C. antarcticus exhibited significantly lower CTmax and heat coma temperatures Ibrutinib than individuals acclimated at either −2 °C or +4 °C, while summer acclimatised individuals of A. antarcticus only showed significantly lower CTmax and heat coma temperatures than individuals maintained at +4 °C. Across all temperatures between −4 and 20 °C, both collembolan species were significantly more active and travelled a greater distance than the mite (P < 0.05 Kruskal–Wallis

test, 4 °C acclimation, Fig. 3). In all species KU-57788 previously acclimated at +4 °C, movement increased with temperature up to 25 °C (except at 9 °C in M. arctica), before decreasing again at temperatures ⩾30 °C. Following an acclimation period at −2 °C (0 °C for M. arctica), there was no significant difference in locomotion at temperatures ⩽0 °C, except for M. arctica, in which movement was significantly greater at −4 °C (P < 0.05 Tukey’s multiple range test, variances not equal) ( Fig. 3). At 15 and 20 °C, movement was most rapid in C. antarcticus acclimated at −2 °C, as compared with the two other acclimation groups. The movement of M. arctica, acclimated at 0 °C, was also more rapid at 20 °C. Individuals of both collembolan species given an acclimation period at +9 °C exhibited considerably

slower movement at temperatures above +4 °C than individuals maintained at +4 °C. In contrast, movement was greater across all temperatures between 0 and 25 °C in +9 °C acclimated individuals mafosfamide of A. antarcticus. There were no significant differences in the SCPs of the three species when maintained at +4 °C (Table 1, P < 0.05 Kruskal–Wallis test). Alaskozetes antarcticus was the only species to show a bimodal distribution. In all three species, the SCPs of individuals acclimated at −2 °C for one month, and summer acclimatised individuals of C. antarcticus and A. antarcticus, were significantly lower than those of individuals maintained at +4 °C (P < 0.05 Kruskal–Wallis test). Conversely, the SCP of individuals after a +9°C acclimation period was not significantly different to those maintained at +4 °C (P > 0.05 Kruskal–Wallis test). Summer acclimatised individuals of C. antarcticus also had significantly lower SCPs than individuals acclimated at −2 °C (P < 0.

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