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“In the post-genomic era, the phage display technology surfaces as an alternative approach for large-scale study of tissue-specific protein interactions with direct clinical and therapeutic applications The unbiased identification of molecular complexes expressed on the surface of cells and blood vessels of organs and tissues may eventually lead to
a considerably improved understanding of cellular and vascular proteomics. The ultimate value of this technology consists in the conception of a new ligand-directed pharmacology, Selleck PSI-7977 with broad implications for both treatment and molecular imaging of cancer patients In this review, we describe the use and applications of phage display for efficient development of targeted drug discovery and design.”
“One objective of preclinical VX-765 price animal models of stroke is to distinguish behavioral compensation
from behavioral recovery. In compensation, a new behavior is substituted for a lost behavior, whereas in recovery, the original behavior is restored. Distinguishing between these processes is important because: (1) compensation can be mistaken for recovery, (2) compensatory strategies can disrupt performance, (3) the behavioral methods, therapy, and neural changes associated with enhancing compensation can be different from those associated with recovery, (4) under different conditions both compensation and recovery can be desirable outcomes. The review describes a behavioral method for assessing hand use in reaching (skilled
reaching or reach-to-eat) by the rat, a behavior analogous to single handed prehension in humans. The method consists of seven separate assessments obtained with end point, movement notation, and biometric measures. The method highlights the importance of using multiple measures to identify behavioral change either during acute, early, and chronic poststroke periods. Distinguishing between compensation and recovery refines the interpretation of preclinical behavioral findings and expands opportunities for developing therapies for stroke. (C) 2013 Elsevier Ltd. All rights reserved.”
“Expression of retroviral replication enzymes (Pol) requires a controlled translational recoding event to bypass the stop codon at the end of gag. This recoding event occurs either by direct suppression of termination via the insertion of an amino acid at the stop codon (readthrough) or by alteration of the mRNA reading frame (frameshift). Here we report the effects of a host protein, large ribosomal protein 4 (RPM), on the efficiency of recoding. Using a dual luciferase reporter assay, we found that transfection of cells with a plasmid encoding RPL4 cDNA increases recoding efficiency in a dose-dependent manner, with a maximal enhancement of nearly twofold.