2% for flat- and 4.2% for protruded-type. This was significantly higher in the depressed-type lesions. The rate of adenomatous component was 6.2%, 50.8% and 55.7%, respectively. This was significantly lower in depressed-type
lesions, and suggested that they emerge directly from the normal epithelium without going through the adenoma stage. Conclusion: Depressed-type colorectal carcinomas are small, but invade massively. They had higher risks of vessel permeation, tumor budding and nodal metastasis than flat- and protruded-types. Furthermore, they had a lower rate of adenomatous component, suggesting that they are “de novo” carcinomas. For their rapid growth and malignant nature, whether or not they are depressed-type Rapamycin is very important in the diagnosis of colorectal neoplasms. Key Word(s): 1. cororectal carcinoma; Presenting Author: XIAOHUI GUAN Additional Authors: XIAOYING LI, XIAO HAN, LIPING AN Corresponding Author: XIAOHUI GUAN Affiliations: Affiliated Hospital of Beihua University; College of Pharmacy of Beihua University Poziotinib Objective: Polyclonal antibody preparation of ASPP1 and research ASPP1 and expression in the colon cancer tissue, study the expression and the colon cancer tissue differentiation degree, infiltration depth,
lymph node metastasis, to clarify the role of ASPP1 in knot bowel cancer. The role of bowel cancer. Methods: According Branched chain aminotransferase to bioinformatics software analysis of ASPP1 protein secondary structure, antigenicity and hydrophilicity, hydrophobicity, physical and chemical properties by homology search, antibody design consideration of other factors, to design a polypeptide, preparation of polyclonal antibody. Using the ELISA and Western blot and immunohistochemistry method to measure its titer and specificity. Using immunohistochemical
Sp method in 30 cases of colon cancer tissue and 15 cases of normal colon tissue expression of ASPP1 and determine the expression of ASPP1 and colon tissue differentiation degree, infiltration depth, lymph node metastasis and clinical pathological features of relations. Results: Successfully predicted its antigenicity analysis; Preparation of polyclonal antibody against people ASPP1; By using ELISA and Western blot and immunohistochemistry staining method confirmed the high antibody titer, the specificity is strong. ASPP1 in carcinoma tissue of high, medium and low differentiation degree of expression of the lower level of differentiation, the less the expression. The infiltration depth, and presence of expression there was no significant difference in lymph node metastasis. ASPP1 in colon cancer and normal colon tissues was no significant difference (p > 0.05). Conclusion: Using bioinformatics software to predict ASPP1 antigenicity, and successful preparation of the high specificity of ASPP1 polyclonal antibody.