Aims: The aim of this study was to investigate duration of IFX infusions, use of premedication and in centres who had changed infusion duration whether there was an increase in side-effects

or loss of clinical response. We also assessed whether routine measurement of anti-infliximab antibodies (ATIs) or IFX trough (IFXT) levels were being used to guide treatment. Methods: We performed a telephone survey during the period March to May 2013. We included all major public hospitals within Australia where children with CD were regularly being treated with IFX. The paediatric gastroenterology (PG) fellow/registrar or PG nurse specialist was contacted. Results: The response rate was 88% (N = 8). Across Australia there is significant variation in infusion duration. The median infusion time in units studied was 2 hours (range 1.5 hours- 4 hours). All centres either regularly or always used premedication with corticosteroids. The single buy PD0332991 centre using 90 minute infusions did not report an increase in side effects or loss of clinical response. No unit was routinely using ATIs or IFXT levels to guide dosage. Conclusion: Wide variation exists in the practice of IFX administration in paediatric CD. The impact these variations have

on outcomes is uncertain. Ideally before changing to the more rapid ‘adult style’ Midostaurin ic50 protocol, a prospective study should be undertaken to assess safety, efficacy and the role of specific biochemical monitoring. G SEIBOTH, D MOORE, R COUPER, P HAMMOND, S BARRY, S KRITAS Gastroenterology Unit, Women’s & Children’s Hospital, WCHN, North Adelaide SA, Australia Introduction: Gastroesophageal reflux (GOR) is a very common condition in children. Currently the standard test for measuring reflux is the 24 h pH probe. A number of studies have investigated the advantage of adding impedance versus pH alone for symptom association however the advantage

for diagnostic yield of reflux without symptom association is yet to be established. The aim of this study was to investigate the diagnostic yield of adding impedance to 24 h pH testing for GOR. Methods: A retrospective analysis of 24 h Dolutegravir datasheet pH/impedance studies completed at the Women’s and Children’s Hospital between 2008 and May 2013 was performed. Studies were categorised according to the following criteria: negative or positive for pH only if total reflux index was >5%, negative or positive for impedance only if total number of episodes >75, negative or positive for both. Results: One hundred and twenty three 24 h pH/impedance studies were included (mean age 4.4 ± 0.5 years, 44% male). Sixty five studies were negative for GOR both by pH only and pH/impedance analysis. Twenty studies were positive for pH only and 16 studies were positive for both pH and impedance. Twenty two studies were found positive for only impedance increasing the diagnostic yield from 41% to 89% (p = 0.006).