All authors read and approved the final manuscript.”
“Background Campylobacteriosis in the most selleck inhibitor common foodborne
disease in European countries, with an overall incidence of 47.6 cases per 100,000 population [1]; in Canada, with 36.1 cases every 100,000 person-years [2]; and the third most important bacterial foodborne diseases in the US [3]. Campylobacter spp. are found still at high prevalence in retail broiler carcasses in the US [4; 5], and the isolation of Campylobacter spp. from clinical and food samples has always been done using microaerobic conditions, generally 85% N2, 10% CO2 and 5% O2, during the enrichment of the selleck samples and during the incubation of plate media. Different methods have been developed to generate microaerobic atmospheres and for a small number of samples, sachets that generate CO2 are commonly used [6].
If a larger number of samples are processed weekly, the evacuation-replacement is a more economical alternative. In this system, the air in the jar is partially removed by a vacuum pump and then replaced with a microaerobic gas mix. For a large number of samples, or to create unique microaerobic gas mixes with increased H2 content, Lazertinib more sophisticated microaerobic workstations have been developed [7]. Besides generating microaerobic conditions, several O2-quenching agents have been traditionally added to enrichment broths and agar plates for the isolation of Campylobacter spp. These agents neutralize the toxic effects of oxygen radicals and include blood or alkaline hematin [8; 9], charcoal [10], iron salts and norepinephrine [11], and ferrous sulfate, sodium metabisulfite and sodium pyruvate (known as FBP supplement) [12]. In general, if blood or charcoal is added to agar plates, no other O2 quenching compounds are added [9]. To ensure the
microaerobic gas mix for the length of incubation (at least 48 h) sealed jars are commonly used, although plastic bags utilized to freeze food products with a “”ziplock”" type closing to prevent air leaks have been successfully used with gas-generating sachets and manual Arachidonate 15-lipoxygenase evacuation-replacement systems [13; 14]. Although a microaerobic mix is indispensable to grow Campylobacter spp. on agar plates, we have long suspected that no extra addition of any microaerobic gas mix is needed to keep Campylobacter spp. alive or even grow them in enrichment broths. In the present study we evaluated 108 retail broiler meat samples and compared the efficacy of Bolton broth incubated under microaerobic conditions using an evacuation-replacement system (subsamples M) versus incubation under aerobic conditions (subsamples A) for the isolation of naturally occurring Campylobacter spp. Presumptive Campylobacter spp. collected on agar plates were confirmed and identified with multiplex polymerase chain reaction (mPCR) assays and their DNA relatedness was analyzed using pulsed-field gel electrophoresis (PFGE).