Alteration of intestinal permeability and tight junction (TJ) is considered a key player due to development of PI-IBS. The aim of this study was to determine whether probiotics could normalize the intestinal epithelial barrier and expression of

TJ proteins such as occludin, claudin-1 and zonula occludens-1 (ZO-1). Methods: Visceral hypersensitivity was induced by Trichinella spiralis infection in mice. At 8 weeks post infection (PI), mouse were gavaged daily for 7 days Temozolomide molecular weight with different strains of probitics respectively (Befidobacterium longum, Lactobacillus acidophilus, Streptococcus faecalis or mixture of the three). Plasma levels of diamine oxidase (DAO) and d-lactate were determined using an enzymatic spectrophotometry. Expression of occludin, claudin-1 and ZO-1 in ileum was determined by Western blotting. Results: 1) Plasma DAO level significantly

increased in PI-IBS group (14.25 ± 2.49 U/ml) compared with control group (8.80 ± 1.64 U/ml, p < 0.05). After administration of Bif.longum, Lac.acidophilus and mixture, plasma DAO levels were decreased Bioactive Compound Library concentration obviously while there is no change in group of Strep.faecalis; 2) Similarly, plasma d-lactate were reduced in groups of Bif.longum, Lac.acidophilus and mixture but group of Strep.faecalis has no reduction; 3) in ileum, claudin-1 in PI-IBS (0.87 ± 0.24) was lower than that of control group (1.21 ± 0.26, p < 0.05). No difference was observed in occludin and ZO-1 between control group and PI-IBS group. In the comparison of PI-IBS, claudin-1 of groups of Bif.longum, Lac.acidophilus and mixture were significantly increased in ileum. Farnesyltransferase Interestingly, expression of claudin-1 of group of mixture was higher than control but other groups were not.; 4) There were significant negative correlations between claudin-1 and DAO (r2 = 0.875, P < 0.05). Conclusion: Befidobacterium longum and Lactobacillus acidophilus but not Streptococcus faecalis, can improve the intestinal permeability of PI-IBS. The mixture

of the three is the most effective. The close correlation between expression of tight junction and plasma levels of DAO supports the hypothesis that probiotics normalize intestinal permeability after infection because of increase in the expression of TJ proteins. Key Word(s): 1. PI-IBS; 2. Probiotics; 3. TJ protein; Presenting Author: LEIJIA LI Additional Authors: JIN TAO, SHENGLIANG CHEN Corresponding Author: LEIJIA LI Affiliations: The Third Affiliated Hospital of Sun Yat-Sen University; Renji hospital, Shanghai Jiaotong university, school of medicine Objective: To construct the lentiviral vector encoding alkB gene and investigate its effect on proliferation and activity of human gastric cancer cells.