Copyright (C) 2010 John Wiley & Sons, Ltd.”
“Danshensu, chemical name D-(+)beta-(3,4-dihydroxyphenyl) lactic acid, is the major water-soluble components of Radix Salviae Miltiorrhizae (Danshen). Sodium danshensu is a sodium salt of danshensu. The purpose of this study is to develop a sensitive, rapid and specific ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method for determination of sodium click here danshensu in rat plasma and exploration of Q-TOF application to quantitative measurement of drugs in vivo by regulating parameter

settings including profile, centriod, high resolution and extended dynamic range. The developed method was validated in terms of linearity, sensitivity, selectivity, decision limit, detection capability, accuracy, and precision. Limit of quantitation was estimated in 1 ng/mL. The excellent MS qualitative and quantitative abilities are integrated. It is able to complete structural identification and quantitative measurement of

a sample.”
“BACKGROUND:

Carotid barochemoreceptor pathological lesions have been studied in animals, but few human necropsies have been performed. Therefore, data rely on case patients following surgery, radiotherapy and carotid endarterectomy. Almost no data are BMS202 concentration available regarding whether the effect of aging prevails over pathological conditions, despite the classic description that glomic fibrosis learn more increases with age.OBJECTIVE:

To morphometrically characterize the alterations of the carotid barochemoreceptors and their supplying arteries.METHODS:

Patients (n=23) who had suffered and died from stroke, with and without complicated internal carotid atheromatosis, were divided

by age (group 1: older than 80 years; group 2: 65 to 80 years; and group 3: younger than 65 years). Carotid segments were obtained at autopsy. The specimens were stained for light microscopy and immunohistochemistry.RESULTS:

Carotid glomus presented from moderate-to-severe atrophy and fibrosis. A focal decrease in vascularization (CD34-positive) of the glomus (greater than 50%) was observed in areas of atrophy and fibrosis. Damaged nerve endings (S100 protein-positive) were observed at the media of the carotid sinus. Morphometric data showed no differences between groups for glomus area, number of type 1 and 2 cells, and the wall to lumen arteriole ratio. No statistical differences were demonstrated in the pathological findings of the carotid glomus when comparing complicated with noncomplicated plaques or age groups.CONCLUSION:

Severe carotid chemoreceptor damage exists in patients who have died from stroke and suffered from carotid atheromatosis. These findings were independent from aging and plaque type. However, damage was correlated with a marked narrowing of the supplying arterioles as a consequence of hemodynamic and/or metabolic alterations (dyslipidemia, diabetes).