In most but not all studies, elevated levels of MVs of endothelial origin are reported in plasma from ACS patients compared to non-ACS patients.[95] and [96] To which extent these endothelial MVs contribute to the hypercoagulable status of these patients, however, is unknown. MVs isolated from blood of patients with essential
thrombocythemia, a chronic myeloproliferative disease that is characterized by an increased risk of both arterial and venous thrombosis, are mostly derived from platelets and ECs. The MVs in these patients are thought to contribute to the hypercoagulable state that is observed in vivo.97 Plasma from patients with certain types of cancer contains higher numbers of vesicles than plasma from healthy subjects.[13], [14] and [98] Furthermore, MVs exposing coagulant TF in blood of cancer patients have been associated not only Stem Cell Compound Library order with thrombosis but also with disease progression.[13] and [15] Interestingly, in some cancer patients Alectinib cost with a detectable level of coagulant TF
present within the blood, a minor fraction of MVs exposes the epithelial marker, MUC-1.13 To which extent these MUC-1-expressing vesicles, i.e. vesicles likely to originate from the tumor, are exposing coagulant TF and to which extent such vesicles are associated with development of VTE, however, remain to be determined.99 Furthermore, tumor cells may elicit a host response that leads to expression of TF by monocytes and possibly ECs, and
to the shedding of MVs bearing TF. Recently, in a study comprising over 200 cancer patients, we found a subpopulation of vesicles in one patient exposing TF, VE-cadherin (CD144) and E-selectin (CD62e), both specific markers of endothelial origin. How much TF exposed by this subpopulation is coagulant or how TF contributes to coagulation activation in vivo has not been investigated yet (A. Kleinjan, MD, personal communication). One has to bear in mind that TF can also induce angiogenesis and transmembrane signaling, each processes important for cancer growth and development. To which extent vesicle-exposed TF contributes to such functions in cancer patients is unknown. It is still unknown whether exosomes are coagulant. This is a relevant question because most vesicles selleck chemicals llc present in body fluids are within the size range of exosomes rather than of MVs, and thus may have a relatively large contribution to coagulation because formation of tenase and prothrombinase complexes requires a membrane surface which both MVs and exosomes could provide. The membrane surface has to expose negatively charged lipids such as PS to enable the formation of the coagulation factor complexes and the PS can be detected by binding of annexin V. Heijnen et al.20 showed that only a relatively low number of exosomes, supposed to originate from platelets, bound annexin V. Furthermore, MVs but not exosomes bound factor X and prothrombin in this study.