pneumoniae+; P6+ was considered as H. influenzae+. Results of reference tests and qmPCR for Streptococcus pneumoniae (Spn) and Haemophilus influenzae (Hi) applied to bronchoalveolar lavage (BAL)

samples in 156 patients with lower respiratory tract infection BMS202 research buy (A) and in 31 control patients (B). From the 21 patients with conventional (blood culture, BAL culture, or BI 10773 supplier urinary antigen test) tests positive for S. pneumoniae, 20 were positive by qmPCR. In addition 34 cases with no conventional test positive for S. pneumoniae were positive with Spn9802 PCR of which 26 were also positive by lytA PCR. Of the 6 patients with pneumococcal bacteraemia, S. pneumoniae was identified by BAL culture in one case, by urinary antigen test in one case, and by qmPCR and lytA PCR in all the 6 patients. Similarly, among the 9 patients with positive urinary antigen test, S. pneumoniae was identified in 8 by BAL qmPCR and in seven by lytA PCR, and none by BAL culture. H. influenzae was not found in any blood culture but was detected by BAL culture in 31 cases, AZD3965 mw of which 28 also were positive

by qmPCR. Of 44 cases proved negative by culture but positive by qmPCR, 41 were confirmed by fucK PCR. Among the 31 control patients S. pneumoniae and H. influenzae were identified by BAL culture in 2 (6%) and 3 (10%) cases respectively, by qmPCR in 8 (26%) and 11 (35%) cases (Table 3B). Of 7 and 8 cases proved negative by culture but positive with qmPCR for S. pneumoniae and H. influenzae respectively, 2 were positive by lytA PCR for S. pneumoniae and 7 were positive by fucK PCR for H. influenzae. Figure 1 shows the qmPCR copy number of the LRTI patients and controls compared to results by culture, urinary antigen test and lytA PCR. Among the qmPCR positive subjects, the LRTI patients and controls had a similar mean log 10 of copy number 5.69 (standard deviation [SD] 1.53) versus 5.65 (SD 1.63); p = 0.79, for H. influenzae and MRIP 6.31 (SD 1.12) versus 5.93 (SD 0.96); p = 0.36,

for S. pneumoniae). If the cut-off limit for a positive qmPCR result was risen to 105 DNA copies/mL, the positivity rate among the controls would drop from 26% (8/31) to 16% (5/31) for S. pneumoniae and from 35% (11/31) to 19% (6/31) for H. influenzae. Similarly in the patient group the positivity rate would drop from 35% (54/156) to 30% (47/156) for S. pneumoniae and from 46% (72/156) to 20% (31/156) for H. influenzae. Figure 1 Multiplex real-time PCR copy numbers of target organisms in patients and controls. Comparison of PCR copy numbers in the LRTI patients and controls compared with culture, urinary antigen test and gel-based lytA PCR. Table 4 shows the sensitivities and specificities of the qmPCR, with the detection limit of the PCR assay itself and a detection limit of 105 copies/mL.