Conclusion: The identification of various modes of action by which anti-MSP-1 antibodies function against the parasite during erythrocytic development emphasizes the importance of functional assays for evaluating malaria vaccines and may also open new avenues for immunotherapy and vaccine development.”
“Crosslinked polydimethylsiloxane/polyetherimide (PDMS/PEI) composite
membranes were prepared, in which asymmetric microporous PEI membrane prepared with phase inversion AZD4547 method was acted as the microporous Supporting layer in the flat-plate composite membrane. The different function Composition Of the PDMS/PEI composite membranes were;ere characterized by reflection Fourier transform infrared (FTIR) spectroscopy. The surface and section of PDMS/PEI composite membranes were investigated by scanning electron microscope (SEM). The composite membranes prepared
in this work were employed in pervaporation separation of benzene/cyclohexane mixtures. Effects of feed temperature, feed composition, concentration of crosslinking agent oil BMN 673 the separation efficiency of benzene/cyclohexane mixtures were investigated experimentally. In addition, the swelling rate and stableness of composite membrane during long time operation were studied, which should be significant for practical application. The results demonstrated that the pervaporation method could be very effective for separation of the benzene/cyclohexane mixtures. (C) 2009 Wiley, Periodicals, Inc. I Appl Polym Sci 112: 2425-2433, 2009″
“BACKGROUND:
Long QT Syndrome (LQTS) is characterized by corrected QT interval prolongation leading to torsades de pointes and sudden cardiac death. LQTS type 2 (LQTS2) is caused by mutations in the KCNH2 gene, leading to a reduction of the rapidly activating delayed rectifier K(+) current and loss of human ether-a-go-go-related gene (hERG) channel function by different mechanisms. Triggers for life-threatening arrhythmias in LQTS2 are often auditory stimuli.
OBJECTIVES: To screen KCNH2 for mutations in patients with LQTS2 on an electrocardiogram and auditory-induced syncope interpreted as seizures and sudden cardiac death, and Liproxstatin-1 chemical structure to analyze their impact on the channel function in vitro. METHODS: The KCNH2 gene was screened for mutations in the index patients of three families. The novel mutations were reproduced in vitro using site-directed mutagenesis and characterized using the Xenopus oocyte expression system in voltage clamp mode.
RESULTS: Novel KCNH2 mutations (Y493F, A429P and del234-241) were identified in the index patients with mostly typical LQTS2 features on their electrocardiograms. The biochemical data revealed a trafficking defect. The biophysical data revealed a loss of function when mutated hERG channels were coexpressed with the wild type.