Panels B and C are the immunoblots of LPS samples in panel A which were hybridized Crenigacestat supplier against sera from serotype A and B patients, respectively. Lane 4 is the LPS from B. pseudomallei strain MSHR1655 which is rough type and not seroreactive. Lane L is a standard protein ladder. (PNG 152 KB) Additional file 3: Figure S2. Comparison of type A O-antigen biosynthesis clusters. Type A O-antigen is found in four species,
from top to bottom, B. oklahomensis, B. pseudomallei, B. mallei, and B. thailandensis. Red indicates nucleotide homology of 78-100%. The glycosyltransferase gene wbiE (BoklE_010100014785) is truncated in B. oklahomensis E0147 but maintains functional. Conversely, insertion of a thymine into the methyltransferase wbiD relative to B. pseudomallei K96243 removes the functionality of this enzyme in E0147, removing it from
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